Faculty Profile

Dr. Ahmed Jawaad Afzal

Associate Professor

Department Of Biology

Plants employ multiple layers of immunity  to guard against infection. The first layer responds to structures within conserved microbial molecules. The second layer responds to effector proteins, which are pathogen-encoded virulence factors.  These two “branches” of the immune system synergize to provide robust host defense that halts most infections. My current work focuses on understanding the role of the multifunctional protein RIN4, which regulates both branches of the plant immune system.

Title Publication Author Year
Classification and experimental identification of plant long non-coding RNAs Genomics Rai M.I., Alam M., Lightfoot D.A., Gurha P., Afzal A.J. 2018
Expression of plant receptor kinases in E. coli Methods in Molecular Biology Agha M.A., Lightfoot D., Afzal A.J. 2017
Proteomic Profiling and the Predicted Interactome of Host Proteins in Compatible and Incompatible Interactions Between Soybean and Fusarium virguliforme Applied Biochemistry and Biotechnology Iqbal M.J., Majeed M., Humayun M., Lightfoot D.A., Afzal A.J. 2016
Quantification of hydrogen peroxide in plant tissues using Amplex Red Methods Chakraborty S., Hill A.L., Shirsekar G., Afzal A.J., Wang G.-L., Mackey D., Bonello P. 2016
Starvation-Induced Dietary Behaviour in Drosophila melanogaster Larvae and Adults Scientific Reports Ahmad M., Chaudhary S.U., Afzal A.J., Tariq M. 2015
The role of NOI-domain containing proteins in plant immune signaling BMC Genomics Afzal A.J., Kim J.H., Mackey D. 2013
Homo-dimerization and ligand binding by the leucine-rich repeat domain at RHG1/RFS2 underlying resistance to two soybean pathogens BMC Plant Biology Afzal A.J., Srour A., Goil A., Vasudaven S., Liu T., Samudrala R., Dogra N., Kohli P., Malakar A., Lightfoot D.A. 2013
The magnaporthe oryzae effector avrpiz-t targets the RING E3 ubiquitin ligase APIP6 to suppress pathogen-associated molecular pattern-triggered immunity in ricesW OA Plant Cell Park C.-H., Chen S., Shirsekar G., Zhou B., Khang C.H., Songkumarn P., Afzal A.J., Ning Y., Wang R., Bellizzi M., Valent B., Wang G.-L. 2012
The receptor like kinase at Rhg1-a/Rfs2 caused pleiotropic resistance to sudden death syndrome and soybean cyst nematode as a transgene by altering signaling responses BMC Genomics Srour A., Afzal A.J., Blahut-Beatty L., Hemmati N., Simmonds D.H., Li W., Liu M., Town C.D., Sharma H., Arelli P., Lightfoot D.A. 2012
Recombination suppression at the dominant Rhg1/Rfs2 locus underlying soybean resistance to the cyst nematode Theoretical and Applied Genetics Afzal A.J., Srour A., Saini N., Hemmati N., El Shemy H.A., Lightfoot D.A. 2012
Separable fragments and membrane tethering of Arabidopsis RIN4 regulate its suppression of PAMP-triggered immunity Plant Cell Afzal A.J., da Cunha L., Mackey D. 2011
Specific threonine phosphorylation of a host target by two unrelated type III effectors activates a host innate immune receptor in plants Cell Host and Microbe Chung E.-H., Da Cunha L., Wu A.-J., Gao Z., Cherkis K., Afzal A.J., MacKey D., Dangl J.L. 2011
Multigeneic QTL: The laccase encoded within the soybean Rfs2/rhg1 locus inferred to underlie part of the dual resistance to cyst nematode and sudden death syndrome Current Issues in Molecular Biology Iqbal M.J., Ahsan R., Afzal A.J., Jamai A., Meksem K., El-Shemy H.A., Lightfoot D.A. 2009
The nematode resistance allele at the rhg1 locus alters the proteome and primary metabolism of soybean roots Plant Physiology Afzal A.J., Natarajan A., Saini N., Iqbal M.J., Geisler M., El Shemy H.A., Mungur R., Willmitzer L., Lightfoot D.A. 2009
Erratum: Kinetic and thermodynamic study of a chemically modified highly active xylanase from scopulariopsis sp (Applied Biochemistry and Biotechnology)) Applied Biochemistry and Biotechnology Afzal A.J., Bokhari S.A., Siddiqui K.S. 2008
Plant receptor-like serine threonine kinases: Roles in signaling and plant defense Molecular Plant-Microbe Interactions Afzal A.J., Wood A.J., Lightfoot D.A. 2008
Soybean disease resistance protein RHG1-LRR domain expressed, purified and refolded from Escherichia coli inclusion bodies: Preparation for a functional analysis Protein Expression and Purification Afzal A.J., Lightfoot D.A. 2007
Kinetic and thermodynamic study of a chemically modified highly active xylanase from Scopulariopsis sp. Applied Biochemistry and Biotechnology Afzal A.J., Bokhari S.A., Siddiqui K.S. 2007
Three minimum tile paths from bacterial artificial chromosome libraries of the soybean (Glycine max cv. 'Forrest'): Tools for structural and functional genomics Plant Methods Shultz J.L., Yesudas C., Yaegashi S., Afzal A.J., Kazi S., Lightfoot D.A. 2006
The Soybean Genome Database (SoyGD): a browser for display of duplicated, polyploid, regions and sequence tagged sites on the integrated physical and genetic maps of Glycine max. Nucleic acids research. Shultz J.L., Kurunam D., Shopinski K., Iqbal M.J., Kazi S., Zobrist K., Bashir R., Yaegashi S., Lavu N., Afzal A.J., Yesudas C.R., Kassem M.A., Wu C., Zhang H.B., Town C.D., Meksem K., Lightfoot D.A. 2006
Innovative kinetic and thermodynamic analysis of a purified superactive xylanase from Scopulariopsis sp. Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology Afzal A.J., Ali S., Latif F., Rajoka M.I., Siddiqui K.S. 2005
A novel thermodynamic relationship based on Kramers theory for studying enzyme kinetics under high viscosity IUBMB Life Siddiqui K.S., Bokhari S.A., Afzal A.J., Singh S. 2004
A pyramid of loci for partial resistance to Fusarium solani f. sp. glycines maintains Myo-inositol-1-phosphate synthase expression in soybean roots Theoretical and Applied Genetics Iqbal M.J., Afzal A.J., Yaegashi S., Ruben E., Triwitayakorn K., Njiti V.N., Ahsan R., Wood A.J., Lightfoot D.A. 2002
Coupling of surface carboxyls of carboxymethylcellulase with aniline via chemical modification: Extreme thermostabilization in aqueous and water-miscible organic mixtures Biotechnology Progress Bokhari S.A., Afzal A.J., Rashid M.H., Rajoka M.I., Siddiqui K.S. 2002
Two simple and rapid methods for the detection of polymer-degrading enzymes on high-resolution, alkaline, cold, in situ-native (HiRACIN)-PAGE and high-resolution, in situ-inhibited native (HiRISIN)-PAGE Biotechnology Letters Afzal A.J., Bokhari S.A., Ahmad W., Rashid M.H., Rajoka M.I., Siddiqui K.S. 2000
Semester Title Code
Spring Semester 2017-18 Senior Project 1702
Spring Semester 2017-18 Doctoral Thesis Research 1702
Spring Semester 2017-18 Doctoral Thesis Research 1702
Fall Semester 2017-18 Senior Project 1701
Fall Semester 2017-18 Doctoral Thesis Research 1701
Fall Semester 2017-18 Directed Research Project 1701
Summer Semester 2016-17 Doctoral Thesis Research 1603
Spring Semester 2016-17 Doctoral Thesis Research 1602
Spring Semester 2016-17 Master's Thesis II 1602
Spring Semester 2016-17 Directed Research Project 1602
Spring Semester 2016-17 Advance Biochemistry 1602
Spring Semester 2016-17 Biochemistry 1602
Spring Semester 2016-17 Biochemistry 1602
Spring Semester 2016-17 Introductory Biology 1602
Fall Semester 2016-17 Doctoral Thesis Research 1601
Fall Semester 2016-17 Master's Thesis I 1601
Fall Semester 2016-17 Advanced Molecular & Cell Biology 1601
Fall Semester 2016-17 Advanced Methods in Biology 1601
Fall Semester 2016-17 Molecular Biology 1601
Fall Semester 2016-17 Methods in Cell and Molecular Biology 1601
Summer Semester 2015-16 Doctoral Thesis Research 1503
Spring Semester 2015-16 Master's Thesis II 1502
Spring Semester 2015-16 Advance Biochemistry 1502
Spring Semester 2015-16 Biochemistry 1502
Spring Semester 2015-16 Senior Project II 1502
Spring Semester 2015-16 Independent Study 1502
Spring Semester 2015-16 Biochemistry 1502
Spring Semester 2015-16 Introductory Biology 1502
Fall Semester 2015-16 Doctoral Thesis Research 1501
Fall Semester 2015-16 Master's Thesis I 1501
Fall Semester 2015-16 Advanced Molecular & Cell Biology 1501
Fall Semester 2015-16 Advanced Methods in Biology 1501
Fall Semester 2015-16 Senior Project I 1501
Fall Semester 2015-16 Independent Study 1501
Fall Semester 2015-16 Methods in Cell and Molecular Biology 1501
Fall Semester 2015-16 Introductory Biology 1501
Summer Semester 2014-15 Independent Study 1403
Spring Semester 2014-15 Literature in MCB (Seminars) 1402
Spring Semester 2014-15 Advance Biochemistry 1402
Spring Semester 2014-15 Biochemistry 1402
Spring Semester 2014-15 Senior Project II 1402
Spring Semester 2014-15 Biochemistry 1402
Spring Semester 2014-15 Introductory Biology 1402
Fall Semester 2014-15 Directed Research Project 1401
Fall Semester 2014-15 Master's Thesis I 1401
Fall Semester 2014-15 Advanced Molecular & Cell Biology 1401
Fall Semester 2014-15 Senior Project I 1401
  • Past Courses
    Semester Title Code
    Spring Semester 2013-14 Directed Research Project 1302
    Spring Semester 2013-14 Advance Biochemistry 1302
    Spring Semester 2013-14 Biochemistry 1302
    Spring Semester 2013-14 Biochemistry 1302
    Spring Semester 2013-14 Introductory Biology 1302
    Spring Semester 2011-12 Biochemistry 1102
    Spring Semester 2011-12 Seminar in Biology-II 1102
    Spring Semester 2011-12 Biochemistry 1102