Faculty Profile

Dr. Ahmed Jawaad Afzal

Associate Professor

Department Of Biology

Plants employ multiple layers of immunity  to guard against infection. The first layer responds to structures within conserved microbial molecules. The second layer responds to effector proteins, which are pathogen-encoded virulence factors.  These two “branches” of the immune system synergize to provide robust host defense that halts most infections. My current work focuses on understanding the role of the multifunctional protein RIN4, which regulates both branches of the plant immune system.

Classification and experimental identification of plant long non-coding RNAsGenomicsRai M.I., Alam M., Lightfoot D.A., Gurha P., Afzal A.J.2018
Expression of plant receptor kinases in E. coliMethods in Molecular BiologyAgha M.A., Lightfoot D., Afzal A.J.2017
Proteomic Profiling and the Predicted Interactome of Host Proteins in Compatible and Incompatible Interactions Between Soybean and Fusarium virguliformeApplied Biochemistry and BiotechnologyIqbal M.J., Majeed M., Humayun M., Lightfoot D.A., Afzal A.J.2016
Quantification of hydrogen peroxide in plant tissues using Amplex RedMethodsChakraborty S., Hill A.L., Shirsekar G., Afzal A.J., Wang G.-L., Mackey D., Bonello P.2016
Starvation-Induced Dietary Behaviour in Drosophila melanogaster Larvae and AdultsScientific ReportsAhmad M., Chaudhary S.U., Afzal A.J., Tariq M.2015
The role of NOI-domain containing proteins in plant immune signalingBMC GenomicsAfzal A.J., Kim J.H., Mackey D.2013
Homo-dimerization and ligand binding by the leucine-rich repeat domain at RHG1/RFS2 underlying resistance to two soybean pathogensBMC Plant BiologyAfzal A.J., Srour A., Goil A., Vasudaven S., Liu T., Samudrala R., Dogra N., Kohli P., Malakar A., Lightfoot D.A.2013
The magnaporthe oryzae effector avrpiz-t targets the RING E3 ubiquitin ligase APIP6 to suppress pathogen-associated molecular pattern-triggered immunity in ricesW OAPlant CellPark C.-H., Chen S., Shirsekar G., Zhou B., Khang C.H., Songkumarn P., Afzal A.J., Ning Y., Wang R., Bellizzi M., Valent B., Wang G.-L.2012
The receptor like kinase at Rhg1-a/Rfs2 caused pleiotropic resistance to sudden death syndrome and soybean cyst nematode as a transgene by altering signaling responsesBMC GenomicsSrour A., Afzal A.J., Blahut-Beatty L., Hemmati N., Simmonds D.H., Li W., Liu M., Town C.D., Sharma H., Arelli P., Lightfoot D.A.2012
Recombination suppression at the dominant Rhg1/Rfs2 locus underlying soybean resistance to the cyst nematodeTheoretical and Applied GeneticsAfzal A.J., Srour A., Saini N., Hemmati N., El Shemy H.A., Lightfoot D.A.2012
Separable fragments and membrane tethering of Arabidopsis RIN4 regulate its suppression of PAMP-triggered immunityPlant CellAfzal A.J., da Cunha L., Mackey D.2011
Specific threonine phosphorylation of a host target by two unrelated type III effectors activates a host innate immune receptor in plantsCell Host and MicrobeChung E.-H., Da Cunha L., Wu A.-J., Gao Z., Cherkis K., Afzal A.J., MacKey D., Dangl J.L.2011
Multigeneic QTL: The laccase encoded within the soybean Rfs2/rhg1 locus inferred to underlie part of the dual resistance to cyst nematode and sudden death syndromeCurrent Issues in Molecular BiologyIqbal M.J., Ahsan R., Afzal A.J., Jamai A., Meksem K., El-Shemy H.A., Lightfoot D.A.2009
The nematode resistance allele at the rhg1 locus alters the proteome and primary metabolism of soybean rootsPlant PhysiologyAfzal A.J., Natarajan A., Saini N., Iqbal M.J., Geisler M., El Shemy H.A., Mungur R., Willmitzer L., Lightfoot D.A.2009
Erratum: Kinetic and thermodynamic study of a chemically modified highly active xylanase from scopulariopsis sp (Applied Biochemistry and Biotechnology))Applied Biochemistry and BiotechnologyAfzal A.J., Bokhari S.A., Siddiqui K.S.2008
Plant receptor-like serine threonine kinases: Roles in signaling and plant defenseMolecular Plant-Microbe InteractionsAfzal A.J., Wood A.J., Lightfoot D.A.2008
Soybean disease resistance protein RHG1-LRR domain expressed, purified and refolded from Escherichia coli inclusion bodies: Preparation for a functional analysisProtein Expression and PurificationAfzal A.J., Lightfoot D.A.2007
Kinetic and thermodynamic study of a chemically modified highly active xylanase from Scopulariopsis sp.Applied Biochemistry and BiotechnologyAfzal A.J., Bokhari S.A., Siddiqui K.S.2007
Three minimum tile paths from bacterial artificial chromosome libraries of the soybean (Glycine max cv. 'Forrest'): Tools for structural and functional genomicsPlant MethodsShultz J.L., Yesudas C., Yaegashi S., Afzal A.J., Kazi S., Lightfoot D.A.2006
The Soybean Genome Database (SoyGD): a browser for display of duplicated, polyploid, regions and sequence tagged sites on the integrated physical and genetic maps of Glycine max.Nucleic acids research.Shultz J.L., Kurunam D., Shopinski K., Iqbal M.J., Kazi S., Zobrist K., Bashir R., Yaegashi S., Lavu N., Afzal A.J., Yesudas C.R., Kassem M.A., Wu C., Zhang H.B., Town C.D., Meksem K., Lightfoot D.A.2006
Innovative kinetic and thermodynamic analysis of a purified superactive xylanase from Scopulariopsis sp.Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and BiotechnologyAfzal A.J., Ali S., Latif F., Rajoka M.I., Siddiqui K.S.2005
A novel thermodynamic relationship based on Kramers theory for studying enzyme kinetics under high viscosityIUBMB LifeSiddiqui K.S., Bokhari S.A., Afzal A.J., Singh S.2004
A pyramid of loci for partial resistance to Fusarium solani f. sp. glycines maintains Myo-inositol-1-phosphate synthase expression in soybean rootsTheoretical and Applied GeneticsIqbal M.J., Afzal A.J., Yaegashi S., Ruben E., Triwitayakorn K., Njiti V.N., Ahsan R., Wood A.J., Lightfoot D.A.2002
Coupling of surface carboxyls of carboxymethylcellulase with aniline via chemical modification: Extreme thermostabilization in aqueous and water-miscible organic mixturesBiotechnology ProgressBokhari S.A., Afzal A.J., Rashid M.H., Rajoka M.I., Siddiqui K.S.2002
Two simple and rapid methods for the detection of polymer-degrading enzymes on high-resolution, alkaline, cold, in situ-native (HiRACIN)-PAGE and high-resolution, in situ-inhibited native (HiRISIN)-PAGEBiotechnology LettersAfzal A.J., Bokhari S.A., Ahmad W., Rashid M.H., Rajoka M.I., Siddiqui K.S.2000